Retuning Rieske-type Oxygenases to Expand Substrate Range
نویسندگان
چکیده
منابع مشابه
Rieske business: structure-function of Rieske non-heme oxygenases.
Rieske non-heme iron oxygenases (RO) catalyze stereo- and regiospecific reactions. Recently, an explosion of structural information on this class of enzymes has occurred in the literature. ROs are two/three component systems: a reductase component that obtains electrons from NAD(P)H, often a Rieske ferredoxin component that shuttles the electrons and an oxygenase component that performs catalys...
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The Rieske nonheme iron aromatic ring-hydroxylating oxygenases (RHOs) NidAB and NidA3B3 from Mycobacterium vanbaalenii PYR-1 have been implicated in the initial oxidation of high-molecular-weight (HMW) polycyclic aromatic hydrocarbons (PAHs), forming cis-dihydrodiols. To clarify how these two RHOs are functionally different with respect to the degradation of HMW PAHs, we investigated their subs...
متن کاملResonance Raman studies of Rieske-type proteins.
Resonance Raman (RR) spectra are reported for the [2Fe-2S] Rieske protein from Thermus thermophilus (TRP) and phthalate dioxygenase from Pseudomonas cepacia (PDO) as a function of pH and excitation wavelength. Depolarization ratio measurements are presented for the RR spectra of spinach ferredoxin (SFD), TRP, and PDO at 74 K. By comparison with previously published RR spectra of SFD, we suggest...
متن کاملA comprehensive phylogenetic analysis of Rieske and Rieske-type iron-sulfur proteins.
The Rieske iron-sulfur center consists of a [2Fe-2S] cluster liganded to a protein via two histidine and two cysteine residues present in conserved sequences called Rieske motifs. Two protein families possessing Rieske centers have been defined. The Rieske proteins occur as subunits in the cytochrome bc1 and cytochrome b6f complexes of prokaryotes and eukaryotes or form components of archaeal e...
متن کاملPerceptual retuning targets features
Introduction Previous work has shown that listeners systematically “retune” their categorical boundaries when presented with real-word tokens containing an ambiguous segment. There have been multiple previous studies on this lexically-guided retuning (Jesse and McQueen 2011; McQueen et al. 2006; Norris et al. 2003), but most work thus far has assumed that the retuning is at the level of the seg...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2011
ISSN: 0021-9258
DOI: 10.1074/jbc.m111.255174